Electrochemical Approach for the In-Situ Synthesis of a Poly(Sulfosalicylic Acid)/Nickel Oxide Nanoparticles/MCM-41 Nanocomposite for Guanine Detection

Guanine, a purine base in deoxyribonucleic acid (dna), has a low redox potential, making it susceptible to oxidation and contributing to dna damage and mutations. in the current study, a new polymer nanocomposite consisting of nickel oxide-mobile crystalline material-41 (nio-mcm-41) nanocomposites and poly(5-sulfosalicylic acid) (pssa) was prepared by electropolymerization for modification of the graphite/glassy carbon electrode (gr/gce) surface and sensitive measurement of the guanine. the synthesized nanocomposite was identified and confirmed by fe-sem, edx and ft-ir techniques. the electrochemical behavior of guanine on the surface of the pssa/nio-mcm-41/gr/gce was analyzed in optimal conditions using cyclic voltammetry (cv) and differential pulse voltammetry (dpv) methods. the proposed modified electrode achieved a satisfactory dynamic range between the anodic peak current and the concentration of guanine, with a linear range of 0.001–36 μm, a sensitivity of 0.977 μa/μm, and the detection limit (lod) was determined to be 0.5 nm (signal/noise = 3). this sensor was successfully used in the determination of guanine in the real sample of fish sperm’s dna.