DNA Binding Study of Dihydropyrano [3, 4-C] Chromene Derivative by some Spectroscopic Techniques

This study was designed to examine the interaction of dihydropyranochromene derivative, 2-amino-4-phenyl-5-oxo-4h, 5h-pyrano-[3, 2-c] chromene-3-carbonitrile (4-pc) with calf thymus dna (ctdna) by absorption spectroscopy, competitive fluorescence studies, circular dichroism (cd) and viscosity measurements. it was found that 4-pc could interact with ctdna through non- intercalative mode and the intrinsic binding constant, kb, for 4-pc with ctdna was estimated to be 2.74 ×10³ m-1. methylene blue (mb) displacement studies revealed that 4-pc could not replace ctdna bounded mb, which is indicative of groove binding mode of 4-pc with dna. furthermore, 4-pc induced detectable changes in the cd spectrum of ctdna as well as changes in its viscosity, corroborate the above experimental results.