Electroreduction of tacrolimus as immunosuppressant agent at hanging mercury drop electrode: application to determination in pharmaceutical formulation

The adsorptive behavior of the immunosuppressive agent tacrolimus was studied by cyclic and differential-pulse voltammetry on a hanging mercury drop electrode (hmde). the drug was ac-cumulated at hmde and two well-defined peak currents were obtained at -1353 and -1417 mv vs. sce (saturated kcl) in borate buffer (ph 10.0) + 0.1 kcl solution. a voltammetric procedure was developed for the determination of tacrolimus using differential-pulse adsorptive stripping voltammetry (dpadsv). the optimum working conditions for determination of the drug were es-tablished. the analysis of tacrolimus in pharmaceutical dosage forms was carried out satisfactori-ly.