Cytotoxic and Apoptogenic Properties of 2-Phenylthia-zole-4- carboxamide Derivatives in Human Carcinoma Cell Lines

Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. caspases, a family of cysteine-dependent aspartate-directed proteases, play a critical role in the initiation and execution of apoptosis.in this study,cytotoxicity and apoptogenic effect of 2-phenyl 4-carboxamide derivatives were eva-luated in sknmc(human neuroblastoma), mcf-7(human breast adenocarcinoma) and ht-29(human colon cancer) cell lines. in cell viability was determined by mtt assay. also, activation of caspase-3 was evaluated by spectrophotometry. the overall cytotoxicity profiles of deriva-tives demonstrated that the ht-29 cell line has more sensitivity respect to other cell lines. moreo-ver, our observations indicated that 3-f and 2-f derivatives and 4-cl derivative increased cas-pase-3 activation in three carcinoma cell line compared to control. collectively, these findings suggest that these derivatives are able to induce apoptosis in cancer cell lines.