Antidiabetic Potential Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) Leaf Extract via Antioxidant Activities, Inhibition of α-glucosidase, α- amylase, G-6-Pase by in Vitro Assay

Background: diabetes is a chronic disease characterized by glucose levels and results in impaired insulin secretion. this disorder has triggered oxidative stress and excess free radicals condition. smallanthus sonchifolius is a traditional medicine that acts as a diabetic therapy. this research aims to bring out the antidiabetic and antioxidant potential of s. sonchifolius extract (sse). materials and methods: this study was conducted to measure the qualitative phytochemical identification, antioxidant and anti-diabetic activity of sse. the antioxidant assay was carried out using 2,2-diphenyl-1-picrylhydrazine (dpph)- scavenging activity, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (abts)-scavenging and hydrogen peroxide (h2o2)-reducing activity assays, ferric reducing antioxidant power (frap) potency, while anti-diabetic activity of sse assay was carried out using inhibitory of α-amylase, α- glucosidase, and glucose-6-phosphatase (g-6-pase). results: sse contained phenols, flavonoids, steroids/triterpenoids, saponins, tannins, and alkaloids. the antioxidant and antidiabetic activities of samples were calculated based on median inhibitory concentration (ic50). the ic50 values of sse antioxidant, respectively, were dpph (ic50 = 62.72 μg/ml), abts (ic50 = 61.03 μg/ml), h2o2 (ic50 = 438.36 μg/ml), the highest frap activity was 125.31 μm fe(ii)/μg extract at a concentration level of sse 50 μg/ml. the ic50 values of sse antidiabetic were α-amylase inhibition (ic50 = 37.86 μg/ml), α-glucosidase inhibition (ic50 = 90.41 μg/ ml), and g-6-pase inhibition (ic50 = 98.07 μg/ml), respectively. conclusions: sse has antidiabetic potential through antioxidant activities and α-glucosidase, α-amylase, and g-6-pase inhibition activities.