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Fosfomycin Protects Intestinal Cells from Nuclear Changes Suggestive of Deoxynivalenol-induced Apoptosis
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نویسنده
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gaudio denisa pérez ,mozo joaquín ,martínez guadalupe ,paggi maría b. fernández ,decundo julieta m. ,romanelli agustina ,dieguez susana ,soraci alejandro
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منبع
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journal of reports in pharmaceutical sciences - 2020 - دوره : 9 - شماره : 2 - صفحه:209 -214
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چکیده
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Background: fosfomycin (fos) is a broad-spectrum antibiotic that inhibits cell wall synthesis. it has bactericidal activity against both gram-positive and gram-negative bacteria. fos also promotes phagocytosis, has immunomodulatory effects, and protects against the toxicity caused by other drugs. on the contrary, deoxynivalenol (don) causes cytotoxicity on tissues of rapid growth and fast turnover. objectives: the aim of this study was to determine the percentage of nuclear changes indicative of doninduced apoptosis on intestinal cell cultures (caco-2) and to evaluate the protective effect of fos on mycotoxin-exposed cells. materials and methods: cell cultures were treated as follows: (1) don: 2.8 μg/ml, (2) calcium fos: 580 μg/ml, (3) don 2.8 μg/ml + calcium fos 580 μg/ml, and (4) negative control. nuclear morphology was evaluated in fixed cells stained with 4′,6-diamino-2-phenylindol and then visualized under an immunofluorescence microscope. results: percentages of cells with nuclear changes were significantly higher in cells treated with don (31.53% ± 4.17%) compared to those incubated with the antibiotic in conjunction with the mycotoxin (5.63% ± 4.23%). on the contrary, there were no significant differences between cells incubated with don + fos and cells incubated only with the antibiotic (1.10% ± 1.55%) when compared to the negative control (3.50% ± 0.09%). conclusion: the results from this study showed that don induces nuclear changes suggestive of apoptosis in intestinal cells and that fos can protect cells from dna damage. further studies are needed to determine whether don induces apoptosis only on cells of epithelial origin and to understand the implications of fos protective effect under in vivo conditions.
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کلیدواژه
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Apoptosis ,deoxynivalenol ,fosfomycin ,intestinal cells ,protective effect
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آدرس
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universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. centro de investigación veterinaria de tandil (civetanconicet), Argentina, agencia nacional de promoción científica y tecnológica (anpcyt), Argentina, universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. centro de investigación veterinaria de tandil (civetanconicet), Argentina, centro de investigación veterinaria de tandil (civetanconicet), argentina. universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina, universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. centro de investigación veterinaria de tandil (civetanconicet), Argentina, universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. centro de investigación veterinaria de tandil (civetanconicet), Argentina, universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. comisión de investigaciones científicas de la provincia de buenos aires, Argentina, universidad nacional del centro de la provincia de buenos aires, facultad de ciencias veterinarias, depto. de fisiopatología, Argentina. centro de investigación veterinaria de tandil (civetanconicet), Argentina
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