investigating the expression levels of glutathione peroxidase and glutathione reductase genes in mastectomies women

Introduction: important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; the expression of these genes in these women can play an important role in the treatment of these patients; the aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.procedure: after cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a co2 incubator at 37°c. in order to synthesize cdna, extracted rna molecules were used with the help of fermentaz revert aid tm kit, produced in the united states.results: the concentration of 500 micrograms of nanoparticles caused the death of more than 60% of mcf 7 cells (p< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal hek293 cell line .conclusion: the anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in mcf-7 breast cancer cell line of mastectomy candidates.introduction: important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; the expression of these genes in these women can play an important role in the treatment of these patients; the aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.procedure: after cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a co2 incubator at 37°c. in order to synthesize cdna, extracted rna molecules were used with the help of fermentaz revert aid tm kit, produced in the united states.results: the concentration of 500 micrograms of nanoparticles caused the death of more than 60% of mcf 7 cells (p< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal hek293 cell line .conclusion: the anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in mcf-7 breast cancer cell line of mastectomy candidates.