the effect of low-level laser therapy on differentiation ability of human adipocyte-derived stem cells to keratinocytes

Background: low-level laser therapy (lllt), is a clinically well-established tool for wound healing. in vitro studies have shown that lllt has abiostimulatory effect on mesenchymal lineages and non-mesenchymal lineages of adipose-derived stem cells (adscs) differentiation. the aim of this study was to evaluate the effects of lllt at different energy intensities on differentiation of (adscs) to keratinocyte.methods: adipose tissue were successfully obtained from 45-year-old woman by lipoaspirates and adipose-derived mesenchymal stem cells (adscs) were isolated. adscs were divided into a control group and three low-level laser treated groups irradiated with 0.5 j/cm2, 1 j/cm2 and 2 j/cm2 laser doses using he-ne laser with 632nm wave length in 5 times. all groups cultured with keratinocytes media for 10-days. cell proliferation was eveluated using 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (mtt) assay. adscs developed a polygonal cobblestone shape characteristic of human keratinocytes. specific keratinocyte marker 5 (krt5) were analyzed by real-time pcr. data were analyzed using one-way avova and tukey-kramer hoc test by spss software version 16.results: results of this study demonstrated lllt could significantly affect proliferation of adscs (p<0.05) and promote adscs differentiation to keratinocytes by increasing expression of krt5 at of 1 j/cm2 and 2 j/cm2 laser doses (p<0.01). lllt at density of 0.5 j/cm2 could not promote adscs differentiation significantly.conclusion: considering these findings, lllt could improve current in vitro methods of differentiating adscs to keratinocyte prior to transplantation. thereby lllt can use clinically as promoting skin wound healing.