alfa-glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of haplophyllum acutifolium dc. and ferula haussknechtii wolff ex rech.

Background: α-glucosidase inhibition can significantly prevent glucose uptake after meal, and help controlling adverse effects in diabetics. so, determination of α-glucosidase inhibitory effect and antioxidant activity of the haplophyllum acutifolium and ferula haussknechtii aerial organs was the aim of this study. methods: inhibitory effect of hexane extracts from different organs was investigated spectrophotometricaly in several concentrations at 405 nm wavelength using a microplate reader. antioxidant activity of hexane extracts from various organs was also measured using dpph scavenging and iron reduction tests. results: the highest inhibitory activity of f. haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and ic50 = 0.1 μg/ml) and the most inhibitory activity of h.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and ic50 = 10 μg/ml) and leaf extract (100% inhibition and ic50 = 60 μg/ml). extract of h. acutifolium flower and leaves showed non-competitive inhibition pattern and f. haussknechtii flower showed mixed (competitive-non-competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. the results of antioxidant potential showed ec50 for f. haussknechtii flower and h. acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively. conclusions: the hexane extract of the f. haussknechtii flower, and h. acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-glucosidase, dpph free radical scavenging activities and reducing power. so, they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients.