bioorganic investigation of encapsulated cysteine derivative into polymeric nanocarrier

In this work, the copolymer-based synthesized cysteine-loaded nanocarriers were prepared by a routine protocol, coprecipitation method. it is the first report to investigate the neuroprotective potential and biocompatibility of cysteine derivatives loaded into poly(ethylene glycol)-block-poly(ε−caprolactone) methyl ether (peg-b-pcl). the average size of the polymeric/empty ncs was 89 nm and for polymeric/synthesized derivative of cysteine was 126 nm. the drug loading efficiency was 81%. the concentration of polymeric ncs was 2.1×10^10 particles/ml and the zeta potential of polymeric/empty and polymeric/synthesized derivative of cysteine ncs -5 mv and -11 mv, respectively. biological part of this work was investigated in the sh-sy5y human neuroblastoma cell line using cell viability and toxicity assays. the concentration of polymeric ncs below 1×10^10 particles/ml was described as a zero-point damageable for the cell line. also, the synthesized derivative of cysteine encapsulated into polymeric ncs has more neuroprotective effect as compared to free cysteine at lower concentration, and therefore, has a significant neuroprotective potential against z-vad-fmk and st-evoked sh-sy5y cell damage.