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Identification of Agrobacterium tumefaciens Strains by PCR-RFLP Analysis of the 16S-rDNA
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نویسنده
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Al-Karablieh Nihayah ,Khlaif Hamed ,Al-Banna Lama
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منبع
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jordan journal of agricultural sciences - 2006 - دوره : 2 - شماره : 3 - صفحه:209 -104
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چکیده
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16s-rdna analysis of 30 agrobacterium tumefaciens jordanian isolates belonging to 1, 2, 3 and intermediate biotypes, were characterized by the restriction fragment length polymorphism analysis of pcr product. two reference cultures of a. tumefaciens were used for comparison. the profile obtained by the analysis formed three main clusters confirmed the biotyping of the strains, and allocated intermediate biotype with biotype 1 strains as a typical biotype 1strains, also, higher genetic distances were found within biotype 1 strains
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کلیدواژه
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Agrobacterium ,16S-rDNA ,PCR-RFLP ,Dendrogram ,Electrophoresis
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آدرس
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University of Jordan, Faculty of Agriculture, Department of Plant Protection, Jordan, University of Jordan, Faculty of Agriculture, Department of Plant Protection, Jordan, University of Jordan, Faculty of Agriculture, Department of Plant Protection, Jordan
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Authors
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