synthesis and cytotoxic evaluation of 2pyrazoline derivative on leukemia cancer cell line k562

The 2-pyrazoline derivatives have a wide range of biological effects, such as anti-viral, anti-bacterial, anti-fungal, anti-depressant and anti-cancer effects. studies have shown that compounds containing 2-pyrazoline along with another heterocycles may show more effective biological properties. in this study, a 2-pyrazoline derivative with a spiro-indenoquinoxaline ring at c3 position was synthesized by one-pot microwave-assisted method and its chemical structure was confirmed by 1h nmr spectroscopy. the cytotoxic effects of the compound were evaluated on the k562 cell line and phytohemagglutinin-activated peripheral blood mononuclear cells (pha+pbmc) by mtt assay. additionally, the cytotoxic effects of cisplatin on these cells were investigated and compared with those of 2- pyrazoline. the ic50 values obtained from the 2-pyrazoline derivative effects on the k562 cell line and pha+pbmc cells were 45 and 55 μg/ml respectively, while cisplatin inhibited proliferation of the same cells with ic50 value 1.71 and 7.8 µg/ml respectively. the results of this study showed that the synthesized derivative had a cytotoxic activity on the k562 cancer cell line at higher concentrations than cisplatin.