Difference in ergosterol concentrations extracted from demethylation inhibitor resistant and susceptible strains of Monilinia fructicola: understanding the ‘Mona’ element

Monilinia fructicola causes disease on peaches and nectarines, and is an economic concern in the south eastern united states. this experiment was designed to try and understand the mechanisms that allow m. fructicola to be resistant to demethylation inhibitor (dmi) fungicides, which is an interest among agronomists. isolates of m. fructicola were analyzed with high performance liquid chromatography to measure differences in ergosterol concentration between selected groups of isolates. this was done to determine if m. fructicola is using an alternate sterol other than ergosterol as the final end product of the sterol biosynthesis pathway, or is using a different enzyme other than 14-a demethylase, to synthesize ergosterol. this experiment showed differences in ergosterol concentration between the groups of isolates that were analyzed. the three groups analyzed in this study included isolates without the ‘mona’ element while susceptible to dmi fungicides, isolates with the ‘mona’ element and resistant to dmi fungicides, isolates with the ‘mona’ element while susceptible to dmi fungicides. statistical analysis revealed significant differences between the three groups of isolates, which suggesting that m. fructicola uses a different enzyme, other than 14-a demethylase, to synthesize ergosterol.

Difference in ergosterol concentrations extracted from demethylation inhibitor resistant and susceptible strains of Monilinia fructicola: understanding the ‘Mona’ element

AbstractMonilinia fructicola causes disease on peaches and nectarines, and is an economic concern in the South Eastern United States. This experiment was designed to try and understand the mechanisms that allow M. fructicola to be resistant to Demethylation Inhibitor (DMI) fungicides, which is an interest among agronomists. Isolates of M. fructicola were analyzed with High Performance Liquid Chromatography to measure differences in ergosterol concentration between selected groups of isolates. This was done to determine if M. fructicola is using an alternate sterol other than ergosterol as the final end product of the sterol biosynthesis pathway, or is using a different enzyme other than 14a demethylase, to synthesize ergosterol. This experiment showed differences in ergosterol concentration between the groups of isolates that were analyzed. The three groups analyzed in this study included isolates without the ‘Mona’ element while susceptible to DMI fungicides, isolates with the ‘Mona’ element and resistant to DMI fungicides, isolates with the ‘Mona’ element while susceptible to DMI fungicides. Statistical analysis revealed significant differences between the three groups of isolates, which suggesting that M. fructicola uses a different enzyme, other than 14a demethylase, to synthesize ergosterol.