ultrasound-assisted extraction coupled with solid-phase extraction coupled with dispersive liquid–liquid microextraction for determination of enrofloxacin in chicken meat

Ultrasound-assisted extraction combined with solid-phase extraction combined with dispersive liquid–liquid microextraction (uae-spe-dllme) has been developed as a new approach for the extraction of enrofloxacin in chicken meat prior to high performance liquid chromatography with uv detection. in the uae-spe-dllme, enrofloxacin was first extracted from 1.0 g chicken meat into the mixture of 10 ml acetonitrile acidified with formic acid buffer (ph=4) and 10 ml edtea-mcilvaine buffer (0.1 m) and 20 ml n-hexane as an extracting phase by using ultrasound-assisted extraction. then, the extract by ultrasound-assisted extraction was used for solid-phase extraction. after clean-up, enrofloxacin was preconcentrated by using dllme technique. thus, 1.5 ml methanol extract (disperser solvent) and 200 μl chloroform (extraction solvent) were added to 5.0 ml ultrapure water and a dllme technique was applied. under the optimum conditions, the linearity of the method was in the range from 10 to 500 μg kg^-1 with the correlation coefficient (r^2) of 0.9972. the method detection limit was 5.0 μg kg^-1. the proposed method has been successfully applied to the analysis of the enrofloxacin in chicken meat, and a satisfactory result was obtained.