alterations of mir-4772-3p and mir-3173-3p expression in tissue compared to normal tissue by real-time pcr

Background: in recent years, many studies have focused on the dysregulation of mirna expression in cancer. these structures act as oncogenes and suppressors. in the present study, the expression levels of mir-3173-3p and mir-4772-3p were investigated in the tissue of glioblastoma multiforme (gbm) patients and healthy individuals. methods: the present study was performed on 50 patients with gbm and 50 healthy individuals as the control group. real-time pcr technique was used to determine the tissue levels of mir-3173-3p and mir-4772-3p. the biomarker evaluation of mir-3173-3p and mir-4772-3p was assessed using roc curve analysis. results: the expression level of mir-4772-3p was higher in women with gbm (p-value = 0.03). increased concentration of mir-3173-3p in tissue of gbm patients showed a significant correlation with a final grade of disease progression (grades iii and iv) (p-value = 0.007). roc curve analysis and cut-off of 0.6939 showed that mir-4772-3p could detect the presence of malignant gbm with 88.89 sensitivity and 100.0 specificity. conclusions: the present study reveals the potential oncogenic role of mir-4772-3p. moreover, the progression of glioblastoma cancer in the final stages can be assessed by measuring the level of mir-4772-3p in patients’ tissue.