evaluation of aβ deposits in the hippocampus of a rat model of alzheimer’s disease after intravenous injection of human adipose derived stem cells by immuno- and thioflavin s-costaining

Background: alzheimer’s disease (ad) is a progressive neuropsychiatric disorder that gradually impairs memory and behavioral functions. amyloid beta (aβ) is considered as the most toxic substance in the brain of ad patients. objectives: the present study was designed to evaluate aβ deposits by immuno- and thioflavin s-costaining in the hippocampus of a rat model of ad after intravenous injection of human adipose-derived stem cells (hadscs). methods: thirty-two male rats were included in the four groups of control, sham, ad and hadscs. the hadscs characterization was confirmed by the flow cytometry technique. immuno- and thioflavin s-costaining was utilized for detecting aβ plaques in the hippocampus of a rat model of ad following injection of hadscs. results: statistical analysis revealed that aβ plaques increased significantly in the ad group compared to the control and sham groups. the administration of hadscs significantly decreased immunoreactivity and thio-s-positive plaques in the ad group. we also found that the plaques detected by anti-aβ antibody (immunohistochemical staining) were significantly more than those dis- tinguished by thioflavin-s in all the groups. conclusions: results showed that hadscs played an effective role in decreasing amyloids aggregation following migration to the hippocampus of the rat model of ad.