effect of casein phosphopeptide-amorphous calcium phosphate (cpp-acp) on bond strength of a universal adhesive to demineralized dentin

Objective: this study investigated the effect of casein phosphopeptide‑amorphous calcium phosphate (cpp‑acp) on the shear bond strength (sbs) of a universal adhesive in self-etch mode to demineralized dentin.methods: seventy-five dentin specimens were randomly divided into five groups (n=15) as follows: group 1, intact dentin; group 2, demineralized dentin receiving no remineralization agent; and groups 3-5, demineralized dentin receiving cpp-acp for 30, 90, and 180 seconds, respectively. all groups were bonded by adper single bond universal adhesive in self-etch mode. after 24 hours, the sbs test was conducted, and failure modes were recorded. the data were analyzed by one-way anova, tukey post-hoc test, and chi-square test at the significance level of p<0.05.results: the highest and lowest mean sbs values were observed in groups 1 (intact dentin: 29.58 ± 8.13 mpa) and 2 (demineralized dentin: 13.41 ± 4.85 mpa), respectively. anova revealed a significant difference in sbs among the groups (p< 0.001). a pairwise comparison revealed that the sbs of demineralized groups (groups 2-5) was significantly lower than that of the intact samples (p< 0.05). group 5, with cpp-acp, applied for 180 seconds, showed a significantly higher sbs (20.74 ± 4.54 mpa) compared to group 2 (p< 0.05).conclusions: applying cpp-acp paste for three minutes can increase the bond strength of the universal adhesive in self-etch mode to demineralized dentin, whereas shorter application times are not effective.