effect of adding cpp-acp into a daily-use toothpaste on remineralization of enamel white spot lesions

Objective: the purpose of this study was to investigate the effect of adding cpp-acp into a daily-use toothpaste on the remineralization of enamel caries lesions.methods: thirty enamel blocks were obtained from bovine incisors. each specimen was divided into three equal parts. one-third of each block was coated with varnish to serve as a sound control area, while the remaining two-thirds underwent a demineralization process. after demineralization, another one-third of the surface was varnished, leaving only one-third of the enamel to undergo remineralization. the enamel blocks were divided into three groups (n=10), according to the remineralization treatment applied as follows: group 1: fluoride-containing toothpaste, group 2: cpp-acp-containing toothpaste, and group 3: fluoride- and cpp–acp–containing toothpaste.  remineralization was assessed through the vickers microhardness test at various depths (20, 50, 120 and 200 µm). the data were analyzed by anova and lsd test, and p< 0.05 was considered statistically significant.results: there was a significant difference in remineralization efficacy between the groups at the depth of 20 µm (p 0.001). pairwise comparisons revealed that the toothpaste containing both fluoride and cpp-acp had a significantly greater microhardness than other experimental groups (p 0.05). no significant difference was observed between the study groups concerning microhardness at 50, 120 and 200 µm depths (p 0.05).conclusions: cpp-acp can serve as a suitable alternative to fluoride in daily-use toothpaste for enamel remineralization. the concurrent use of fluoride and cpp-acp in toothpaste can generate a synergistic remineralizing effect at the enamel surface layer.