in vitro cytotoxicity of silver nanoparticles incorporated in a soft silicone liner

Introduction: silver nanoparticles (snps) have recently been suggested to increase the antimicrobial properties of soft liner materials. however, their safety remains a matter of debate. this study aimed to evaluate the cytotoxicity of mucopren® soft silicone liner material (mucopren; kettenbach, germany) incorporated in snps. methods: the snps with over 98% purity were added to mucopren in 0.5, 1, 2, and 3 weight percentage (wt%) concentrations and manually homogenized. the mixture of the pieces of mucopren plus snps and snps alone were placed in 96-well plates containing dulbecco's modified eagle medium culture, fbs, and antibiotics with l929 fibroblasts. cell viability and biocompatibility were determined after 1, 2, and 3 days of incubation using the methylthiazol tetrazolium assay. optical density was read by an elisa reader at 570 nm and compared to those of positive and negative controls. results: among mucopren mixed with different snps concentration, the cell toxicity had no significant difference in the same days, and cell toxicity decreased over time (p=0.016). the snps alone were less cytotoxic than mucopren incorporated snp samples (p>0.05). conclusion: within the limitations of this study, the addition of 0.5, 1, 2, and 3wt% concentrations of snps to mucopren did not cause a significant change in its cell toxicity in an in vitro condition.