تاثیر غلظت های مختلف اسید سالیسیلیک بر بیان ژن استیلبن سنتاز و تولید رزوراترول در انگور رقم سلطانی

اﻫﺪاف: ﻣﺘﺎﺑﻮﻟﯿﺖﻫﺎی ﺛﺎﻧﻮﯾﻪ ﮔﯿﺎﻫﯽ ﺑﻪﺳﺒﺐ ﺗﻨﻮع و ﻧﻘﺸﺸﺎن در ﮔﯿﺎﻫﺎن و ﺳﻼﻣﺘﯽ اﻧﺴﺎن ﻣﻮرد ﺗﻮﺟﻪ ﻗﺮار ﮔﺮﻓﺘﻪاﻧﺪ. اﻧﮕﻮر ﯾﮑﯽ از ﮔﯿﺎﻫﺎن ﺑﺎ ﺗﺮﮐﯿﺒﺎت دوم و ﺑﺎ ﺧﻮاص داروﯾﯽ اﺳﺖ. اﯾﻦ ﺗﺮﮐﯿﺒﺎت ﺷﺎﻣﻞ رزوراﺗﺮول اﺳﺖ ﮐﻪ ﺗﺮﮐﯿﺒﯽ ﻓﻨﻠﯽ از ﮔﺮوه اﺳﺘﯿﻠﺒﻨﻮﺋﯿﺪﻫﺎﺳﺖ. ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﻓﺰاﯾﺶ ﺗﻮﻟﯿﺪ ﭘﻠﯽﻓﻨﻞ رزوراﺗﺮول ﺗﺤﺖ ﺗﺄﺛﯿﺮ اﻟﯿﺴﯿﺘﻮرﻫﺎ، آزﻣﺎﯾﺸﯽ ﺑﺎ ﻃﺮح ﺗﺼﺎدﻓﯽ ﺑﺎ 4 ﺗﮑﺮار در اﻧﮕﻮر رﻗﻢ ﺳﻠﻄﺎﻧﯽ اﻧﺠﺎم ﺷﺪ. ﻣﻮاد و روشﻫﺎ: ﻫﻮرﻣﻮن اﺳﯿﺪﺳﺎﻟﯿﺴﯿﻠﯿﮏ ﺑﻪﻋﻨﻮان اﻟﯿﺴﯿﺘﻮر در ﻏﻠﻈﺖﻫﺎی ﻣﺨﺘﻠﻒ ﺻﻔﺮ، 4-^10 ، 5-^10 و 6-^10 ﻣﻮﻻر ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ و ﺑﻪ ﻣﺤﯿﻂ ﮐﺸﺖ ms ﺑﺪون ﻫﻮ رﻣﻮن ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺗﻨﺶ اﺿﺎﻓﻪ ﺷﺪ. در ﺳﻄﺢ ﻣﻮﻟﮑﻮﻟﯽ، ﺗﺎﺛﯿﺮ اﺳﯿﺪﺳﺎﻟﯿﺴﯿﻠﯿﮏ در ﺑﯿﺎن ژن اﺳﺘﯿﻠﺒﻦ ﺳ ﻨﺘﺎز ﺗﻮﺳﻂ rt-pcr ﻧﯿﻤﻪ ﮐﻤﯽ ارزﯾﺎﺑﯽ ﺷﺪ. در آزﻣﺎﯾﺶ ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ، ﻣﯿﺰان ﺗﻮﻟﯿﺪ رزوراﺗﺮول ﺗﻮﺳﻂ دﺳﺘﮕﺎه hplc اﻧﺪازهﮔﯿﺮی ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: آﻧﺎﻟﯿﺰ ﺑﯿﺎن ژن اﺳﺘﯿﻠﺒﻦﺳﻨﺘﺎز ﻧﺸﺎن داد ﮐﻪ اﺳﯿﺪﺳﺎﻟﯿﺴﯿﻠﯿﮏ ﺑﺎ ﻏﻠﻈﺖ4-^10 ﻣﻮﻻر ﺗﺄﺛﯿﺮ ﻣﺜﺒﺖ و اﻓﺰاﯾﺸﯽ در ﺑﯿﺎن ژن داﺷﺘﻪ و اﻓﺰاﯾﺶ 35/48 درﺻﺪی در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺷﺎﻫﺪ ﻧﺸﺎن داد و ﻫﻤﭽﻨﯿﻦ ﻏﻠﻈﺖ 5-^10 ﻣﻮﻻر ﺑﯿﺎن ژن را 5/65 درﺻﺪ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺷﺎﻫﺪ اﻓﺰاﯾﺶ داد. در آزﻣﺎ ﯾﺶ ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ اﻓﺰاﯾﺶ در ﻣﯿﺰان ﺗﻮﻟﯿﺪ رزوراﺗﺮول در ﺗﯿﻤﺎر 4-^10 ﻣﻮﻻر در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻣﺸﺎﻫﺪه ﺷﺪ (6/10 ﻣﯿﮑﺮوﮔﺮم) و ﺗﯿﻤﺎر5-^10 ﻣﻮﻻر (3/25 ﻣﯿﮑﺮوﮔﺮم) ﺗﻔﺎوت ﻣﻌﻨﯽداری ﺑﺎ ﻧﻤﻮﻧﻪ ﺷﺎﻫﺪ ﻧﺸﺎن ﻧﺪاد.ﻧﺘﯿﺠﻪﮔﯿﺮی: اﺳﯿﺪﺳﺎﻟﯿﺴﯿﻠﯿﮏ ﺑﻪﻋﻨﻮان ﯾﮏ اﻟﯿﺴﯿﺘﻮر و ﻣﺤﺮک ﺗﻮﻟﯿﺪ رزوراﺗﺮول ﻣﯽﺗﻮاﻧﺪ ﺑﯿﺎن ژن اﺳﺘﯿﻠﺒﻦ-ﺳﻨﺘﺎز و در ﭘﯽ آن ﺧﻮاص داروﯾﯽ ﮔﯿﺎه اﻧﮕﻮر را اﻓﺰاﯾﺶ دﻫﺪ.

The Effect of Different Concentrations of Salicylic Acid on Expression of Stilbene Synthase Gene and Production of Resveratrol in Grape cv. Soltani

Aims The secondary metabolites of plants have been considered due to their diversity and roles in plants and human health. Grapevine is one of the plants that have secondary compounds with medicinal properties. such compounds include resveratrol which is a phenolic compound from the stilbenoid group. In order to investigate the resveratrol production under the effect of an elicitor, a CRD design with four replications using Soltani cultivar was done.Materials Methods. The salicylic acid (SA) was used as an elicitor with variable concentrations including 0, 104, 105, 106 and was introduced into MS medium without hormones to examine it`s stress effects. At the molecular level, the effect of SA on the expression of stilbenesynthase gene was evaluated by semiquantitative RTPCR. In the biochemical experiment, the rate of resveratrol production was measured by HPLC.Findings Stilbenesynthase expression analysis showed that SA with a concentration of 104 M had a positive and incremental effect on gene expression and showed a 35.48% of increase compared to the control state, and also the concentration of 105 M increased the gene expression 5.65% in relation with control state. In the biochemical experiment, increasing in production of resveratrol was observed in 104 M treatment compared to the control treatment (6.1 micro;g) and 105 M treatment (3.25 micro;g) did not show a significant difference with the control sample.Conclusion SA as an elicitor and stimulant of resveratrol production could enhance the expression of stilbenesynthase gene followed by enhancing the medicinal properties of the Vitis plant.