سنتز و شناسایی نانوذرات زیست سازگار کیتوسان/آمینوتترازول بعنوان نانوحامل جدید جهت تحویل ژن

در اﯾﻦ ﻣﻘﺎﻟﻪ ﺗﻼش ﺷﺪه اﺳﺖ ﺗﺎ ﺑﺎ اﺳﺘﻔﺎده از 5-آﻣﯿﻨﻮ-1h-ﺗﺘﺮازول و ﺑﯿﻮﭘﻠﯿﻤﺮ ﮐﯿﺘﻮﺳﺎن در ﺟﻬﺖ ﺳﻨﺘﺰ ﯾﮏ ﻧﺎﻧﻮ ﺣﺎﻣﻞ ﺟﺪﯾﺪ و زﯾﺴﺖ ﺳﺎزﮔﺎر ﺑﺎ ﮐﺎراﯾﯽ ﺑﺴﯿﺎر ﺑﺎﻻ و ﮐﻤﺘﺮﯾﻦ ﺳﻤﯿﺖ در اﻧﺘﻘﺎل ژن اﺳﺘﻔﺎده ﺷﻮد. اﯾﻦ ﻧﺎﻧﻮﺣﺎﻣﻞ ﺟﺪﯾﺪ، ﻋﻼوه ﺑﺮ ﻏﯿﺮﺳﻤﯽ و زﯾﺴﺖ ﺗﺨﺮﯾﺐ ﭘﺬﯾﺮ و ﻣﻘﺮون ﺑﺼﺮﻓﻪ ﺑﻮدن، ﺑﺴﯿﺎر ﮐﺎرآﻣﺪ و دارای ﺗﻮاﻧﺎﯾﯽ ﺑﺎﻻ در ﻣﺤﺎﻓﻈﺖ از ژن ﻣﯽ ﺑﺎﺷﺪ. ﺳﺎﺧﺘﺎر و ﺗﺮﮐﯿﺐ ﺷﯿﻤﯿﺎﯾﯽ ﻧﺎﻧﻮﺣﺎﻣﻞ ﺳﻨﺘﺰ ﺷﺪه، ﺑﻄﻮر ﮐﺎﻣﻞ ﺗﻮﺳﻂ آﻧﺎﻟﯿﺰﻫﺎیfesem, tem, xrd, ftir, zeta potential و dls ﺑﺮرﺳﯽ و ﺷﻨﺎﺳﺎﯾﯽ ﺷﺪ. ﻫﻤﭽﻨﯿﻦ، ﺟﻬﺖ ﺑﺮرﺳﯽ ﺗﻮاﻧﺎﯾﯽ ﻧﺎﻧﻮﺣﺎﻣﻞ ﻣﻮرد ﻧﻈﺮ در اﻧﺘﻘﺎل ژن ﺑﻪ داﺧﻞ ﺳﻠﻮل، از رده ﺳﻠﻮﻟﯽ hek-293t اﺳﺘﻔﺎده ﮔﺮدﯾﺪ.

Synthesis and characterization of biocompatible chitosan/aminotetrazol nanoparticles as a novel nanocarrier for gene delivery

This article has been attempting to present a novel and biocompatible nanocarrier based on chitosan biopolymer with high efficiency and low toxicity for gene delivery. At the first, 5amino1Htetrazol reacted with (3chloropropyl)trimethoxysilane and then, the obtained organosilane intermediate was used for functionalization of chitosan amino groups. The structure and chemical composition of the synthesized nanocarrier were fully characterized by FESEM, TEM, XRD, FTIR, Zeta potential and DLS analysis. At the fallowing, the MTT test was performed in order to investigation of the nanocarrier toxicity. Furthermore, Hek293T cell line was used for investigation of the nanocarrier efficiency in gene transfection toward the cell. The loading capacity of plasmid was optimal at an N/P ratio of 3 with excellent protection of the plasmid. The results were shown that the novel nanocarrier is high efficient and has high potential to gene transfection and protection in addition of nontoxicity, biodegradability and cost effectively.