ﺑﺮرﺳﯽ ﺧﺎﺻﯿﺖ آﻧﺘﯽﺑﺎﮐﺘﺮﯾﺎل ﺗﺮﮐﯿﺐ ﻧﺎﻧﻮذرات ﻧﻘﺮه ﺳﻨﺘﺰﺷﺪه ﺗﻮﺳﻂ ﻋﺼﺎره ﻗﺎرچ ﮔﺎﻧﻮدرﻣﺎ و ﮐﺎرﺑﺮد آن در ﺟﺪاﯾﻪﻫﺎی اﺷﺮﯾﺸﯿﺎﮐﻠﯽ ﺟﺪاﺷﺪه از ﻋﻔﻮﻧﺖﻫﺎی ادراری ﻣﻘﺎوم ﺑﻪ ﭼﻨﺪﯾﻦ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ

ﻋﻔﻮﻧﺖ ﻣﺠﺎری ادراری از راﯾﺞﺗﺮﯾﻦ ﻋﻔﻮﻧﺖﻫﺎی ﺑﺎﮐﺘﺮﯾﺎﯾﯽ اﺳﺖ و ﺗﻌﺪاد زﯾﺎدی از ﻣﺮاﺟﻌﻪﮐﻨﻨﺪﮔﺎن ﺑﻪ ﭘﺰﺷﮏ و ﺑﯿﻤﺎران ﺑﺴﺘﺮی در ﺑﯿﻤﺎرﺳﺘﺎنﻫﺎ را ﮐﺴﺎﻧﯽ ﺗﺸﮑﯿﻞ ﻣﯽدﻫﻨﺪ ﮐﻪ اﯾﻦ ﻣﺸﮑﻞ را دارﻧﺪ. ﻧﺎﻧﻮذرات در زﻣﯿﻨﻪﻫﺎی ﻣﺨﺘﻠﻒ ﭘﺰﺷﮑﯽ و ﺻﻨﻌﺘﯽ ﮐﺎرﺑﺮد دارﻧﺪ و ﻧﺎﻧﻮذرات ﻧﻘﺮه ﺑﻪدﻟﯿﻞ رﺳﺎﻧﺎﯾﯽ ﺧﻮب و ﭘﺎﯾﺪاری ﺷﯿﻤﯿﺎﯾﯽ از اﻫﻤﯿﺖ ﺑﺴﯿﺎری ﺑﺮﺧﻮردار ﻫﺴﺘﻨﺪ. ﻧﺎﻧﻮذرات ﻧﻘﺮه ﺑﺎ آزادﺳﺎزی ﯾﻮنﻫﺎی ﻧﻘﺮه ﻋﻠﯿﻪ ﺑﺎﮐﺘﺮیﻫﺎی ﮔﻮﻧﺎﮔﻮن اﺛﺮﺑﺨﺶ اﺳﺖ. اﯾﻦ ﻣﻄﺎﻟﻌﻪ روی50 ﻧﻤﻮﻧﻪ از ﮐﺸﺖﻫﺎی ﻣﺜﺒﺖ ﻣﺒﺘﻼ ﺑﻪ ﻋﻔﻮﻧﺖ ادراری ﻣﺮاﺟﻌﻪﮐﻨﻨﺪﮔﺎن ﺑﻪ آزﻣﺎﯾﺸﮕﺎه ﺑﯿﻤﺎرﺳﺘﺎن اﻣﺎم رﺿﺎ (ع) ﺑﺠﻨﻮرد اﻧﺠﺎم ﺷﺪ ه اﺳﺖ . ﻣﻘﺎوﻣﺖ و ﺣﺴﺎﺳﯿﺖ ﺟﺪاﯾﻪﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از روش اﻧﺘﺸﺎر دﯾﺴﮏ ارزﯾﺎﺑﯽ و اﺛﺮات ﺿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻧﺎﻧﻮذرات ﻧﻘﺮه ﺑﺎ اﺳﺘﻔﺎده از ﻋﺼﺎره آﺑﯽ ﻗﺎرچ ﮔﺎﻧﻮدرﻣﺎ ﻟﻮﺳﯿﺪوم ﺑﻪ روش ﻣﯿﮑﺮوداﯾﻠﻮﺷﻦ )رﯾﺰ رﻗﺖ ﺳﺮﯾﺎﻟﯽ( اﻧﺠﺎم ﺷﺪ ﺑﺮای اﻧﺪازهﮔﯿﺮی اﺑﻌﺎد و ﺷﮑﻞ ﻧﺎﻧﻮذرات ﻧﻘﺮه از ﻣﯿﮑﺮوﺳﮑﻮپ اﻟﮑﺘﺮوﻧﯽ روﺑﺸﯽ اﺳﺘﻔﺎده ﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﺑﺮای ﺑﺮرﺳﯽ ﺗﺮﮐﯿﺒﺎت آﻟﯽ اﺣﺘﻤﺎﻟﯽ ﮐﻪ در ﺳﻨﺘﺰ ﻧﺎﻧﻮذرات اﻣﮑﺎن ﻣﺪاﺧﻠﻪ داﺷﺘﻨﺪ، آﻧﺎﻟﯿﺰ ﻃﯿﻒﺳﻨﺠﯽ ﻣﺎدون ﻗﺮﻣﺰ اﻧﺠﺎم ﺷﺪ. ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ ﻣﺮﺑﻮط ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ آﻣﭙﯽ ﺳﯿﻠﯿﻦ ( 84درﺻﺪ) ﺑﻮد و ﻧﺎﻧﻮذرات اﺑﻌﺎد 20 ﺗﺎ 45 ﻧﺎﻧﻮﻣﺘﺮ داﺷﺘﻨﺪ. ﻣﯿﺎﻧﮕﯿﻦ ﻏﻠﻈﺖ mbc و mic ﺑﻪدﺳﺖآﻣﺪه در اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪﺗﺮﺗﯿﺐ 509/83 و 230 ﺑﻮد. ﻧﺎﻧﻮذرات ﺗﻮﻟﯿﺪﺷﺪه ﺧﺎﺻﯿﺖ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ دارﻧﺪ و در ﻣﻘﺎدﯾﺮ ﻣﻌﯿﻦ ﻣﯽﺗﻮاﻧﻨﺪ ﺟﺎﯾﮕﺰﯾﻦ ﺧﻮﺑﯽ ﺑﺮای درﻣﺎن ﺑﯿﻤﺎریﻫﺎی ﻋﻔﻮﻧﯽ ﻣﻘﺎوم ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎ ﺑﺎﺷﻨﺪ.

Effect of silver nanoparticles on Escherichia coli isolates from urinary tract infections resistant to several antibiotics

AbstractUrinary tract infection is one of the most common and common bacterial infections, accounting for a significant proportion of hospital admissions (about 3040%). Silver nanoparticles work by releasing silver ions against various bacteria. The fact that bacteria are not resistant to nanoparticles is very important and therefore will affect a wide range of bacteria.Materials and MethodsIn this study, 50 specimens of positive cultures with urinary tract infection referred to Imam Reza Hospital Laboratory in Bojnourd were studied. Resistance and susceptibility of the isolates were determined by disk diffusion method. In this study, antibacterial effects of silver nanoparticles were investigated by microdilution method using aqueous extract of Ganoderma leucidum. Vegetative electron microscopy was used to measure the size and shape of silver nanoparticles. In addition, infrared spectroscopy analysis was performed to investigate possible organic compounds involved in the synthesis of nanoparticles.Results: The highest antibiotic resistance was related to ampicillin (84%). The resulting nanoparticles were 20 to 45 nm in size.Conclusion:The produced nanoparticles have antimicrobial activity and can be a good alternative in the treatment of antibiotic resistant infectious diseases.