Ion Selective Membrane Electrodes for the Determination of Xylometazoline Hydrochloride in Rabbit Aqueous Humor using 2-Hydroxy Propyl B-cyclodextrin and Calix[6]arene as Ionophores

Three novel xylometazoline hydrochloride (xylo) selective electrodes wereinvestigated with dioctyl phthalate (dop) as a plasticizer in a polymeric matrix of polyvinylchloride (pvc). sensor 1 was fabricated using phosphomolybdic acid (pma) as an anionicexchanger without incorporation of an ionophore. sensor 2 used 2-hydroxy propyl ?-cyclodextrin(hp?-cd) as an ionophore while sensor 3 was constructed using calix[6]arene as an ionophore.linear responses of xylo within the concentration ranges of 10?4 to 10?2, 10?5 to 10?2 and 10?5 to10?2 mol l?1 were obtained using sensors 1, 2 and 3, respectively. nernstian slopes of 49.78, 56.21and 46.20 mv/decade over the ph range of 5–9 for sensors 1, 2 and 5-7 for sensor 3 wereobserved. the selectivity coefficients of the developed sensors indicated excellent selectivity forxylo. the utility of 2-hydroxy propyl ?-cyclodextrin and calix[6]arene as ionophores had asignificant influence on increasing the membrane sensitivity and selectivity of sensors 2 and 3compared to sensor 1. the proposed sensors displayed useful analytical characteristics for thedetermination of xylo in bulk powder, different pharmaceutical formulations, and biologicalfluids (rabbit aqueous humor) and in the presence of its degradation product (2, 6- dimethyl-4-tertbutyl-phenylacetic acid) and thus could be used as stability-indicating method.