Differential Pulse Voltammetric Assay of Antibacterial Drug Doxycycline Hyclate

The electrochemical oxidation of doxycycline hyclate has been carried out inbritton - robinson buffer at carbon paste and glassy carbon electrodes. doxycycline hyclateexhibits a well-defined irreversible oxidation peak in a broad ph range (2-11). differentialpulse voltammetry was used to determine doxycycline hyclate in pure form. the peakcurrent varied linearly in the following ranges: 2.0×10-7- 3.0×10-6 mol l-1 and 2.0×10-7- 2.6×10-6 mol l-1 in case of carbon paste electrode and glassy carbon electrode, respectively.in case of carbon paste electrode the limits of detection (lod) and quantification (loq) were6.56×10-8 mol l-1 and 2.19×10-7 mol l-1, respectively. for glassy carbon electrode the lodand loq were 9.55×10-8 mol l-1 and 3.18×10-7 mol l-1, respectively. the percentagerecoveries were found in the following ranges: 99.58-101.16% and 99.46-100.98% for carbonpaste electrode and glassy carbon electrode, respectively. the relative standard deviationswere found in the following ranges: 0.53-1.79% and 0.48-1.96% in case of carbon pasteelectrode and glassy carbon electrode, respectively. differential pulse voltammetry wassuccessfully applied for the determination of doxycycline hyclate in pharmaceutical form.