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Three-Dimensionally Microporous Polypyrrole Film as an Efficient Matrix for Enzyme Immobilization
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نویسنده
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Qian Jing ,Peng Lei ,Wollenberger Ulla ,Scheller W. Frieder ,Liu Songqin
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منبع
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analytical and bioanalytical electrochemistry - 2011 - دوره : 3 - شماره : 3 - صفحه:235 -248
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چکیده
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The development of new porous materials has opened unprecedented opportunitiesfor a wide range of applications. in the present work, a three-dimensionally interconnectedporous polypyrrole structure was proposed for biosensing. silica nanoparticles with goodmonodispersion and uniformity of the spheres synthesized by improved st?ber methods wasused as template for porous polypyrrole electrodeposition. voltammetric and electrochemicalimpedance measurements showed that the polypyrrole 3d-network arrays provided excellentmatrices for the immobilization of horseradish peroxidase. the immobilized horseradishperoxidase on these polypyrrole 3d-network arrays retained its bioactivity effectively anddisplayed a pair of redox peaks with a formal potential of -0.275 v and an excellentelectrocatalytic response toward the reduction of hydrogen peroxide (h2o2). this allowed thedetection of h2o2 concentration with a linear range from 1 ?m to 7.94 ?m. the detectionlimit was found to be 0.22 ?m at a signal-to-noise ratio of 3. this method provided analternative way for the biosensor construction.
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کلیدواژه
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Silica Template ,3D-Network Arrays ,Horseradish Peroxidase ,Porou Polypyrrole
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آدرس
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School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, P. R. China, China, Department of Analytical Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Ger, Germany, Department of Analytical Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Ger, Germany, Department of Analytical Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Ger, Germany, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, P. R. China, China
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پست الکترونیکی
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liusq@seu.edu.cn
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Authors
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