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A Novel Amperometric Hydrogen Peroxide Biosensor Based on Catalase Immobilization on Poly(glycidyl methacrylate-co-vinylferrocene)
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نویسنده
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Cevik Emre ,?enel Mehmet ,Abas?yan?k Fatih M.
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منبع
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analytical and bioanalytical electrochemistry - 2011 - دوره : 3 - شماره : 1 - صفحه:14 -25
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چکیده
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A novel amperometric hydrogen peroxide biosensor was fabricated for thedetermination of h2o2. catalase (cat) was immobilized on a glassy carbon electrode bypoly(glycidyl methacrylate-co-vinylferrocene) (poly(gma-co-vfc)). a polymeric electrontransfer mediator, containing copolymers of glycidyl methacrylate (gma) andvinylferrocene (vfc) with different molar ratios, have been prepared by free radicalcopolymerization. amperometric response was measured as a function of concentration ofh2o2, at fixed potential of +0.35 v vs. ag/agcl in a phosphate buffered saline (ph 7.5). themediated hydrogen peroxide biosensor had a fast response of less than 7 s with linear range0.5-14 mm. the sensitivity of the biosensor for h2o2 was 34 na/mm.cm2.
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کلیدواژه
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Catalase ,Hydrogen Peroxide Biosensor ,Redox Polymer ,Mediator ,Ferrocene
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آدرس
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Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey Fatih University, Department of , Turkey, Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey , Turkey , Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey Fatih University, Department of , Turkey
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Authors
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