Incidence and distribution of Tobacco streak virus isolated from parsley Petroselinum sativum

The purpose of this study was to determine the distribution of tobaccostreak virus (tsv) in the vegetable fields of the markazi, qom, lorestan andhamadan provinces. this study was performed in 2017, and a total of 475 samplesof parsley plants were collected. using the specific antibody of the virus, the tsvinfection of these samples was investigated by the immunosorbent assay through thedouble antibody sandwich elisa (das-elisa) method. the results of this studyindicated that the tsv infection of parsley samples in qom, markazi, hamedan andlorestan provinces were 14, 18.8, 15.4 and 20.1%, respectively. also, thephylogenetic analysis of nucleotide and amino acid sequences of the coat protein ofthese isolates showed that iranian and indian isolates could be clustered along witheach other. the phylogenetic tree obtained based on nucleotide and amino acidsequences of the coat protein gene, showed that the isolates were divided into twoand three clusters, respectively. iranian isolates were clustered along with global tsvisolates and other ilarviruses formed a separate cluster. this is the first report of tsvgenetic diversity in iran, and also the first report of tsv infection in the vegetablefields of qom, markazi, hamedan and lorestan provinces.