Evaluation of fluorescence-activated cell sorting technology in agrobacterium biocontrol

Fluorescence-activated cell sorting (facs) as a novel and sensitive technology was used to evaluate the biocontrol efficiency of bacillus subtilis against agrobacterium tumefaciens, a very destructive plant pathogen. the combination of two methods of culturing and cell sorting by facs technology was used to distinguish a rapid and accurate method in monitoring the biocontrol effect of bacillus (atcc21332) on agrobacterium (ibrc-m10701 and agl1),. the culture method indicated that the b. subtilis could suppress a. tumefaciens in vitro and in vivo. we used a green fluorescent protein (gfp), reporter, to flow cytometric analysis using facs. the mean of gfp expression levels was significantly reduced to 17.98, 16.48, and 11.27% in treatments 24, 48, and 72 h post-treatment; however, it was 31.57, 26.06, and 23.98% in the nontreated ones. the experiments demonstrated a positive biocontrol effect of bacillus against agrobacterium. overall, our findings may provide a basis for improving the new rapid biocontrol agent detection method based on facs.