Evaluation of the Anti-inflammatory and Cytotoxic Activity of Methanol Extractsof Psacalium decompositum and Scopulophyla parryi in VERO and Detroit CellLines

Periodontal diseases are characterized by inflammation and destruction of tooth-supporting tissues. there is interest in antiinflammatorysubstances of plant origin because of their advantage in relation to classic anti-inflammatory drugs. thecytotoxic and anti-inflammatory effect of extracts of scopulophila parryi and psacalium decompositum (a.gray) h.rob. &brettell were evaluated with the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium (mtt) method and their proinflammatorycytokine profile was determined in a human peripheral blood monocyte culture model. the plant was collectedand identified and the methanol extract was prepared by maceration. cytotoxicity was evaluated in vitro with the mtt (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium) assay in two cell lines, vero and detroit 551 human fibroblasts. theanti-inflammatory activity of human peripheral blood monocytes and gene expression of the proinflammatory cytokines, il-1, il-6 and il-8, were evaluated the extract of p. decompositum was less toxic on the vero and detroit 551 cell lines witha dl50 of 77.30 μg/ml and the s. parryi extract had 150.58 μg/ml. both extracts significantly reduced expression of il-1β,il-6, and il-8 at 300 μg/ml for s. parryi and at 400 μg/ml for p. decompositum. the extract of p. decompositum was lesstoxic for both cell lines and the two methanol extracts are good alternatives as immunomodulators of proinflammatorycytokines.