Phytochemical Constituents, Antioxidant Activity and Toxicity Potential of Phlomis Olivieri Benth.

Background and objectives: phlomis olivieri benth. (lamiaceae) is a medicinal plant widely distributed in iran. in the present study, we have investigated the phytochemical constituents, antioxidant activity and general toxicity potential of the aerial parts of this species. methods: silica gel (normal and reversed phases) and sephadex lh-20 column chromatographies were used for isolation of compounds from methanol-soluble portion (msp) of the total extract obtained from p. olivieri aerial parts. the structures of isolated compounds were elucidated using 1h-nmr, 13c-nmr and uv spectral analyses. antioxidant activity and general toxicity potential of msp were also evaluated in dpph free radical-scavenging assay and brine shrimp lethality test (bslt), respectively. results: one caffeoylquinic acid derivative, chlorogenic acid (1), one iridoid glycoside, ipolamiide (2), two phenylethanoid glycosides, phlinoside c (3) and verbascoside (5), along with two flavonoids, isoquercetin (4) and naringenin (6) were isolated and identified from msp. the msp exhibited considerable antioxidant activity in dpph method (ic50; 50.4 ± 4.6 µg/ml), compared to bht (ic50; 18.7 ± 2.1 µg/ml), without any toxic effect in bslt at the highest tested dose (1000 µg/ml). conclusion: the results of the present study introduce p. olivieri as a medicinal plant with valuable biological and pharmacological potentials.