Evaluation of Anti-Malarial Activity of Artemisia Turcomanica and A. Kopetdaghensis By Cell-Free B-Hematin Formation Assay

Background and objectives: the plants of genus artemisia (asteraceae) have been conventionally used for prevention and medication of a number of ailments. in the present research, ten extracts with different polarities from aerial parts of two artemisia species, a. kopetdaghensis and a. turcomanica were evaluated for their potential anti-malarial properties. methods: the plant materials were extracted successively with petroleum ether (pe), dichloromethane (dcm), ethyl acetate (etoac), ethanol, and ethanol-water (1:1 v/v) by cold maceration method. cell free b-hematin formation assay were used for assessing anti-malarial activity of obtained extracts. results: dcm extract of a. kopetdaghensis and pe extract of a. turcomanica showed remarkable anti-malarial activity with ic50 values of 1.04±0.02 mg/ml and 0.90±0.27 mg/ml, respectively, compared to positive control (chloroquine, ic50 0.04±0.01 mg/ml). conclusion: it seems that the anti-malarial activity of these extracts might be bound up with the presence of compounds with low or medium polarity; hence, this preliminary test indicated that these potent extracts could be considered for further investigations to find new sources of anti-malarial phytochemicals.