evaluation of wound healing and cytotoxic activities of anacardic acid (13:0) isolated from pistacia vera hull extract

Background and objectives: pistacia vera fruit is a popular nut belonging to anacardiaceae family. traditionally, the hulls have been used as herbal remedies for treatment of oral and skin wounds,peptic ulcers and hemorrhoids. methods: in this study, anacardic acid (13:0) was elucidated by ei-ms, ftir, 1d-nmr and 2d-nmr data analysisfrom active fraction. cytotoxic activity was assessed against normal nih/3t3 cells, and several cancerous human cells, including human breast cancer (mcf-7), hepatocarcinoma (hepg-2) and gastric cancer (mkn-45) using mtt assay. the wound healing activity of this compound was evaluated using in vitro scratch-wound healing assay on nih/3t3 cells. results: anacardic acid (13:0) was toxic at the concentrations tested against all cell lines (6.25-100 µg/ml). theselectivity index showed no selective cytotoxicity (si< 2); however, anacardic acid (13:0) revealed significant wound healing effects through the migration of nih/3t3 cells at the concentrations of 1.25-5 µg/ml. conclusion: these results suggested that anacardic acid (13:0) from p. verahull has cytotoxic activity on human cancer cell lines and can also be useful as a bioactive molecule in wounds treatment. however, more in vitro and in vivo studies need to be done to confirm the efficacy and cytotoxicity of anacardicacid (13:0).