network analysis of 20s-ginsenoside rg3 effect on human colorectal adenocarcinoma cell line ht-29

Background and objectives: 20s-ginsenoside rg3 is a pharmacological active compound of ginseng. evidences indicate that s20-rg3 as an anti-cancer factor plays role in prevention and treatment of cancer. in the present study, proteomic data of 20s-ginsenoside rg3 effect on human colorectal adenocarcinoma cell line ht-29 was analyzed via network analysis to understand more details about the molecular events. methods: the differentially expressed proteins (deps) related to the effect of 20s-ginsenoside rg3 on human colorectal adenocarcinoma cell line ht-29 were extracted from literature and analyzed via protein-protein interaction (ppi) network. the central nodes of the network were determined based on degree value and betweenness centrality. results: eight deps plus 100 added first neighbors were included in the ppi network. five central nodes as hub-bottlenecks including actb, gapdh, tp53, akt1, and alb among the added first neighbors and anxa5 as hub-bottleneck and gstp1 and pcna as bottlenecks among the queried deps were introduced. conclusion: pcna, gstp1, and anxa5 as cell protective proteins are the crucial targeted proteins by 20s-ginsenoside rg3 in the treated cell line ht-29. up-regulation of gstp1 and anxa5 is correspondent to the cell protective property of 20s-ginsenoside rg3, and down-regulation of pcna refers to the opposite effect. it seems that cell protective roles of 20s-ginsenoside rg3 are accompanied with the possible side effects.