molecular identification of gram-negative bacteria in respiratory samples of cystic fibrosis patients from a children referral hospital in tehran

Background: gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (cf).objectives: the study aimed to conduct the molecular identification of gram-negative bacterial flora causing pulmonary infection in children with cf.methods: in this study, sputum samples were taken from 64 cf children undergoing treatment as outpatients or inpatients at a referral children’s hospital in tehran. the pcr technique was used to detect the presence of gram-negative bacteria, namely pseudomonas spp., acinetobacter baumannii, stenotrophomonas maltophilia, and burkholderia cepacia.results: all samples were positive for 16srrna. pseudomonas spp. and a. baumannii were detected in 47% and 14% of the studied samples, respectively. co-colonization by pseudomonas spp. and a. baumannii was observed in three (5%) samples.conclusions: according to this survey, pseudomonas spp. were the most prevalent gram-negative bacteria isolated from cf patients with pulmonary infection by molecular assays.