distribution of uropathogenic virulence genes in escherichia coli isolated from children with urinary tract infection in sanandaj, iran

Background and objectives: uropathogenic escherichia coli (upec) strains are the most common cause of urinary tract infections (utis) in children. upec isolates express a range of virulence traits promoting effective colonization of urinary tract. the aim of this study was to determine antibiotic susceptibility and virulence determinants of upec isolated from children. methods: this cross-sectional study was performed on 32 e. coli strains recovered from urine samples of children with uti aged 0 to 12 years in spring 2015 (between april and june) in sanandaj, iran. the isolates were examined by pcr for the presence of virulence genes encoding haemolysin (hly), cytotoxic necrotizing factor type 1 (cnf 1), p-fimbriae (pap), and afimbrial adhesin (afa). sensitivity to antibiotics was determined using the disk diffusion method. results: the prevalence of genes encoding adhesins was 25% for pap, and 15.6% for afa. the hly and cnf genes encoding toxins were amplified in 15.6% and 25% of isolates, respectively. the strains isolated from hospitalized patients displayed a greater number of virulence genes compared to the isolates from outpatients. different patterns of virulence genes were identified. nitrofurantoin and trimethoprim/sulfamethoxazole were the most and least effective antibiotics with susceptibility rates of 96.9% and 21.9%, respectively. conclusions: these data show the need for monitoring of drug resistance and its consideration in the treatment of e. coli infections. investigation of bacterial pathogenicity associated with uti may help have better medical intervention and management of uti.