Statistical modeling and optimization of cellulase production by Bacillus licheniformis NCIM 5556 isolated from the hot spring,Maharashtra,India

Cellulase shows great interest in the field of organic acids,and biotechnology industries. cellulase-producing thermophilic bacteria was isolated from west coast hot spring (rajapur,ratnagiri district of maharashtra (lat. 16°38′42″n; long. 73°31′53″e) and identified as bacillus licheniformis. out of seven variables in the cellulase production medium,four variables such as carboxy methyl cellulose (cmc),calcium chloride,tween-20,and temperature were screened through plackett–burman design and further optimized through response surface methodology (rsm) for higher cellulase production. the optimal conditions were found to be cmc,19.21 g/l,cacl2·6h2o,25.06 mg/l,tween-20,2.96 ml/l and temperature 43.35 °c. the high cellulase production 42.99 iu/ml was achieved in a 7 l scale bio fermenter using optimal conditions. a 3 fold increase in cellulase production was achieved by rsm model. these methods have proven suitable to optimize cellulase production by a thermophilic bacillus. © 2016 the authors