Fabrication and functionalization of magnesium nanoparticle for lipase immobilization in n-propyl gallate synthesis

An extracellular lipase partially purified from bacillus thermoamylovorans bhk67 was effectively immobilized onto modified magnetic mgfe2o4 nanoparticles (nps). nps were prepared by the sol-gel auto-combustion method and characterized by fourier transform infrared (ftir) spectroscopy,x-ray diffraction (xrd),ultra-violet–visible spectroscopy (uv–vis) and atomic force microscopy (afm). protein loading reached a saturated amount of about 0.20 mg lipase per milligram of mgfe2o4 nps with 78.9% binding efficiency. the nps-bound lipase also showed stability following exposure to n-propanol and iso-propanol or fecl2 and mgcl2 metal ions at (1 mm) at 55 °c. nps-bound lipase also retained 50% of its original hydrolytic activity even after 8th cycle,as well as after 12 h of incubation at 55 °c. nps-bound lipase in an esterification reaction of n-propanol and gallic acid (25 mm) performed for 12 h at 55 °c produced n-propyl gallate with a conversion rate of 82%. synthesized n-propyl gallate possessed strong antioxidant activity,which was confirmed by dpph assay,and in addition has anticancerous activity which was tested on a human l132 cell line. © 2017 the authors