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the characteristics of an ovine lymphoid cell-linesensitive to vaccinal infectious bursal disease virus strain
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نویسنده
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zandieh s ,lotfi m ,kamalzadeh m ,shiri n ,parmour e ,eshaghi a ,masoudi s ,hablolvarid m. h. ,shoushtari a. ,goudarzi h ,taher mofrad s. m. j. ,amanpour s.
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منبع
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archives of razi institute - 2017 - دوره : 72 - شماره : 3 - صفحه:173 -179
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چکیده
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Infectious bursal disease (ibd), also known as gumboro disease, is a globally well-known disease with a significant socio-economic effect. for control of ibd, several commercial egg- and cell-based vaccines are prepared. the cell-based ibd vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. the main cell line used to product the cell-based ibd vaccines, is a primary chicken embryo fibroblast (cef). nevertheless, manipulation of cef is extremely challenging and time-consuming. this study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to who technical report series; no. 978, annex iii. this authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. this cell line passed all defined tests and was considered as a suitable one for ibd vaccine preparation in a large scale.
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کلیدواژه
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infectious bursal disease ,gumboro disease ,chicken embryo fibroblast ,ovine lymphoid origin cell line ,cell characterization
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آدرس
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islamic azad university, ahar branch, faculty of sciences, department of genetics, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of quality control, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of quality control, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of quality control, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of quality control, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of quality control, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of research and production of poultry viral vaccine, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, department of pathology, ایران, agricultural research, education, and extension organization, razi vaccine and serum research institute, ایران, agricultural research,education, and extension organization, razi vaccine and serum research institute, ایران, islamic azad university, tehran medical sciences branch, department of genetics, ایران, tehran university of medical sciences, center of cancer biology research, ایران
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پست الکترونیکی
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saeidamanpour@yahoo.com
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تعیین ویژگی‌های یک سلوللاین گوسفند با منشاء لنفوئیدی حساس به سویه واکسینال ویروس عامل بیماری بورس عفونی طیور
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Authors
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زندیه سکینه ,لطفی محسن ,کمال زاده مرتضی ,شیری نادیه ,پرمور عفت ,اسحاقی علی ,مسعودی شهین ,حبل الورید محمد حسن ,شوشتری عبدالحمید ,گودرزی حسین ,طاهر مفرد سید محمد جواد ,امانپور سعید
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