evaluation of cytokine mrna expression in vaccinated guinea pigs with foot-and-mouth disease type o inactivated vaccine

Foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyzed for quantification of ifn-γ, tnf-α and il-10 expression using relative real-time pcr assay. our results showed that all of the genes wereupregulated. the expression of tnf-α and il-10 genes significantly increased (p<0.05) in day 28th incomparison to the day 7th post the first vaccination. it can be concluded that the vaccine induced immuneresponses by increasing expression of the cytokines. therefore, effects of dna vaccines on immune system alsomay be evaluated using these genes.