Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle

An oil adjuvant vaccine (oav) was prepared from a local strain ofpasteurella multocida. strain 6:b was cultured and inactivated by formalin.bacterial pellet was prepared by centrifugation and subsequently adjuvanted fly montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by 1m route. animals were bled before and at 24, 90, 150, and 200 days post-vaccination. collected sera were used in passive mouse protection test (pmpt). active mouse protection test (ampt) was carried out for oav according to standard method. results of pmpt showed 100% protection up to 150 days and 66-83% up to 200 days post-vaccination. in ampt, 4 log of protection was gained. in this experiment the immunity induced by oav adjuvanted by isa-70 could protect the calves.