Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody

An indirect enzyme-linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain).optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined oiug/ml, 1:3000 and 1:100, respectively. the optical densities (od) were compared with a commercial elisa kit. also, the results of elisa were compared to hemagglutination inhibition (hi) test. the correlation coefficient of the results of both elisas and hi was significant (p<0.05). the developed elisa can detect antibody against ibv and is more sensitive and suitable for screening of samples in diagnostic laboratories.