A serological survey for hydatidosis among buffaloes in Orumia, Iran.

Hydatid cyst is an important and zoonotic infection caused by cystic stage of echinococcus granulosus. it is a major and economic problem in most areas of the world that livestock are kept such as iran. in this study, a total of 111 buffalo's sera from center of buffalo sperm preparation (cbsp), orumia (west azarbayjan province, iran, 2003) were examined for the presence of hydatidosis by using enzyme-linked immunosorbent assay (elisa). 5 ug protein per ml of antigen b from sheep hydatid cyst fluid was used in this assay. optimum dilution for rabbit anti-bovine peroxidase conjugate (sigma) was used 1:5000. overall results indicated 32.4% of serum samples positive for hydatidosis. results indicate buffalo is sensitive intermediate host for echinococcus granulosus in this province.