synthesis of remdesivir derivate as a generation of anti-covid-19 drugs through acetylation reactions

Remdesivir, a competitive inhibitor of viral rna-dependent rna polymerase, is the drug of choice for anti-covid-19 treatment. however, the instability of these substances in plasma raises doubts about their therapeutic potency. additionally, sars-cov-2-infected cells may exhibit a variety of antiviral behaviors due to intricate activation pathways. therefore, this study aimed to develop a synthesis for the remdesivir derivative. the remdesivir derivative was synthesized using acetyl chloride as a reagent in a ratio of 1:3 in dichloromethane and tetrahydrofuran solvent at 30°c for 6 h. thin-layer chromatography and spectrophotometers (1h nmr and 13c nmr) were used to identify the produced molecule, which was a brownish-yellow crystalline powder. the results of the synthesis yielded 0.8 gr (77.34%), and the rf value of the remdesivir derivate was 0.54. the characterization with 1h nmr at δ2.5 ppm (3h, s) indicated the presence of a proton in the h-c-c=o structure caused by the substitution of the acetyl group in the remdesivir structure. the 13c nmr data indicated the presence of aromatic carbons, alkenes, c≡n, and carbon bonds with electronegative o. this remdesivir derivate chemical can be a potential candidate for an anti-covid-19 drug that has more potency because it has substitutions of acetyl groups at positions 2' and 3' in the structure of remdesivir.