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   immunity evaluation of an experimental designed nanoliposomal vaccine containing fmdv immunodominant peptides  
   
نویسنده heshmati l ,rezayat s. m ,madani r ,emami t ,jafari m. r ,golchinfar f ,kazemi m ,azimi dezfouli s. m
منبع archives of razi institute - 2021 - دوره : 76 - شماره : 5 - صفحه:1183 -1190
چکیده    Foot-and-mouth disease (fmd) is a highly contagious viral disease affecting cloven-hoofed animals. the particular virus causing fmd disease is called fmd virus and is a member of the aphthovirus genus in the picornaviridae family. the fmd virus has an 8500 nt long single strain positive rna genome with one open reading frame (orf) trapped in an icosahedral capsid protein. this virus genome doesn’t have proofreading property which leads to high mutagenesis. it has seven serotypes, including o, a, asia, sat1, sat2, and c serotypes, as well as many subtypes. iran is an endemic region for foot-and-mouth disease. vaccination of susceptible animals with an inactivated whole-virus vaccine is the only way to control the epidemic in many developing countries. today, conventionally attenuated and killed virus vaccines are being used worldwide. in iran, animals have been vaccinated every 105 days with an inactivated fmd vaccine. although commercially available fmd vaccines are effective, they provide short-term immunity requiring regular boosters. a new fmd vaccine is needed to improve immunization, safety, and long-term immune responses. a synthetic peptide vaccine is one of the safe and important vaccines. peptide vaccine has low immunogenicity, requiring strong adjuvants. nanoliposomes can be used as new adjuvants to improve immune response. in the current study, nanoliposomal carriers were selected using dimyristoylphosphatidylcholine (dmpc), dimyristoyl phosphoglycerol (dmpg), and cholesterol (chol) as an adjuvant containing two immunodominant synthetic fmdv peptides. the liposomal formulations were characterized by various physicochemical properties. the size, zeta potential, and encapsulation efficiency were optimized, and the obtained nanoliposome was suitable as a vaccine. the efficacy of vaccines has been evaluated in guinea pigs as animal models. indirect elisa was used to detect fmdv-specific igg. the obtained results indicated that although antibody titer was observed, the amount was lower compared to the groups that received inactivated virus-containing liposomes. in addition, the results showed that liposome was an appropriate adjuvant, compared to other adjuvants, such as alum and freund, and can act as a depot and induce an immune response.
کلیدواژه guinean pigs ,adjuvants ,elisa ,encapsulation efficiency
آدرس islamic azad university, tehran medical sciences branch, faculty of advanced sciences and technology, department of medical nanotechnology, iran, islamic azad university , tehran pharmaceutical sciences branch, faculty of pharmacy, department of pharmacology and toxicology, iran. tehran university of medical sciences, school of medicine, school of advanced technologies in medicine, department of nanotechnology in medicine, department of pharmacology, iran, islamic azad university, tehran science and research branch, faculty of veterinary medicine, department of pathobiology, iran. agricultural research education and extension organization (areeo), razi vaccine and serum research institute, department of proteomics and biochemistry, iran, agricultural research education and extension organization (areeo), razi vaccine and serum research institute, department of proteomics and biochemistry, iran, mashhad university of medical sciences, nanotechnology research center, pharmaceutical technology institute, school of pharmacy, department of pharmaceutical nanotechnology, iran, agricultural research education and extension organization (areeo), razi vaccine and serum research institute, department of proteomics and biochemistry, iran, university of guilan, faculty of sciences, department of biology, iran, razi vaccine and serum research institute, department of foot and mouth vaccine production, iran
پست الکترونیکی m.azimi@rvsri.ac.ir
 
   Immunity Evaluation of an experimental designed nanoliposomal vaccine containing FMDV immunodominant peptides  
   
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