aflatoxin m1-binding ability of selected lactic acid bacteria strains and saccharomyces boulardii in the experimentally contaminated milk treated with some biophysical factors

There is a growing concern regarding the recurrent observation of aflatoxins (afs) in the milk of lactating animals. regarding this, the present study was conducted to assess the aflatoxin m1 (afm1)-binding ability of three species, namely lactobacillus rhamnosus, l. plantarum, and saccharomyces boulardii, in afm1- contaminated milk. the mentioned species were administered at the concentrations of 107 and 109 cfu/ml to skimmed milk contaminated with 0.5 and 0.75 ng/ml afm1 within the incubation times of 30 and 90 min at 4°c and 37°c. lactobacillus rhamnosus was found to have the best binding ability at the concentrations of 107 and 109 (cfu/ml), rendering 82% and 90% removal in the milk samples with 0.5 and 0.75 ng/ml afm1, respectively. accordingly, this value at 107 and 109 cfu/ml of l. plantarum was obtained 89% and 82% with 0.75 ng/ml of afm1, respectively. for s. boulardii at 107 and 109 cfu/ml, the rates were respectively estimated at 75% and 90% with 0.75 ng/ml of afm1. the best afm1-binding levels for l. rhamnosus, l. plantarum, and s. boulardii were 91.82±10.9%, 89.33±0.58%, and 93.20±10.9, respectively, at the concentrations of 1×109, 1×107, and 1×107 cfu/ml at 37, 4, and 37°c, respectively. in this study, the maximum afm1 binding (100.0±0.58) occurred while a combination of the aforementioned probiotics was employed at a concentration of 1×107 cfu/ml at 37°c with 0.5 ng/ml afm1, followed by the combination of l. rhamnosus and l. plantarum (95.86±10.9) at a concentration of 1×109 cfu/ml at the same temperature with 0.75 ng/ml afm1. it was concluded that the use of s. boulardii in combination with lactobacillus rhamnosus and l. plantarum, which bind afm1 in milk, can decrease the risk of afm1 in dairy products.