|
|
|
|
molecular detection of anaplasma marginale and anaplasma ovis (rickettsiales: anaplasmataceae) in ixodid tick species in iran
|
|
|
|
|
|
|
|
نویسنده
|
hosseini-chegeni a. ,tavakoli m. ,goudarzi gh. ,telmadarraiy z. ,sharifdini m. ,faghihi f. ,ghanbari m.k.
|
|
منبع
|
archives of razi institute - 2020 - دوره : 75 - شماره : 1 - صفحه:39 -46
|
|
چکیده
|
The present study was conducted as the first molecular detection of anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of iran. a total of 130 tick specimens were collected from hormozgan, lorestan, and guilan, iran, within 2015 to 2017. hyalomma asiaticum, hyalomma dromedarii, rhipicephalus sanguineus, and rhipicephalus (boophilus) species were identified in different geographical regions. an amplicon of 464-bp msp4 of anaplasma was amplified using polymerase chain reaction in various tick species. three sequences, including one anaplasma marginale from r. (boophilus) species and two anaplasma ovis from rhipicephalus sanguineus, were obtained after sequencing. it is concluded that bovine and ovine anaplasmosis agents are present in tick samples in iran. the use of the gene families of six major surface proteins for the detection of various anaplasma species is recommended.
|
|
کلیدواژه
|
anaplasma ,phylogenetic tree ,msp4 ,iran ,tick
|
|
آدرس
|
lorestan university, faculty of agriculture, department of plant protection, iran, agricultural research, education and extension organization (areeo), lorestan agricultural and natural resources research center, iran, lorestan university of medical sciences, department of medical microbiology, iran, tehran university of medical sciences, school of public health, department of medical entomology and vector control, iran. high education institute of rahyan novin danesh (rnd), irn, guilan university of medical sciences, school of medicine, department of medical parasitology and mycology, iran, iran university of medical sciences, cellular and molecular research center, iran. lorestan university of medical sciences, razi herbal medicines research center, iran, iran university of medical sciences, school of health management and information sciences, department of national program of zoonotic disease, iran
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
تشخیص مولکولی آناپلاسما مارجیناله و آناپلاسما اویس (ریکتزیالس: آناپلاسماتاسئا) در گونههای کنه سخت ایران
|
|
|
|
|
Authors
|
|
|
Abstract
|
|
|
Keywords
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|