effects of magnesium sulfate administration on testicular ischemia/reperfusion injury in rats

This study aimed at investigating the effects of intraperitoneal (ip) administration of magnesium sulfate (mgso4) on testicular ischemia-reperfusion (ir) injury in rats. in total, 50 adult wistar rats were randomly divided into 5 groups. group 1 received no injection (control); however, group 2 was subjected to 2 h of i and 24 h of r. subsequently, group 3 was subjected to 2 h of 1, and after 1 h of i, 125 mg/kg mgso4 was injected intraperitoneally followed by 24 h of r. groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of mgso4, respectively. after 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. according to the results, there was a significant increase in tissue malondialdehyde (mda) among i/r rats (p<0.05), whereas mgso4 decreased i/r-induced mda (p<0.05). furthermore, experimental i/r diminished glutathione peroxidase (gpx) and superoxide dismutase (sod) levels significantly (p<0.05). moreover, mgso4 (250 and 500 mg/kg) increased gpx and sod activity significantly in i/r rats (p<0.05). furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the i/r rats. regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of mgso4 (250 and 500 mg/kg)-treated experimental i/r-induced rats. in conclusion, this study demonstrated the beneficial effects of mgso4 on testicular ir injury in rats.