|
|
|
|
Genotyping of Different Pestiviruse Isolates by RT-PCR and RFLP techniques
|
|
|
|
|
|
|
|
نویسنده
|
Kargar Moakhar ,Akhavizadegan M.A. ,Hemmatzadeh F. ,Amini F.
|
|
منبع
|
archives of razi institute - 2004 - دوره : 58 - شماره : 1 - صفحه:1 -8
|
|
چکیده
|
1320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in ag-elisa assay. all these 39 samples in addition to 5 cytopathic strains were cultured in md-bk cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 bvdv suspected isolates were detected by rt-pcr using a primer set. differentiation among the viruses was achieved by cutting the pcr products with restriction endonucleses enzymes ava1, bgl1 and alu1. using this procedure it was possible to distinguish at least two genogroups, 1 and 2 bdv containing 14 and 3 isolates, respectively
|
|
کلیدواژه
|
Bovine viral diarrhea ,RT-PCR ,RFLP ,genotyping
|
|
آدرس
|
Razi Vaccine and Serum Research Institute, Animal Viral Diseases Research & Diagnosis Dep, ایران, Razi Vaccine and Serum Research Institute, Microbiology Dept, university of tehran, Faculty of Veterinary Medicine, Microbiology Dept, ایران, university of tehran, Faculty of Veterinary Medicine, Aquatic Animal Health Disease Dept, ایران
|
|
پست الکترونیکی
|
rokarm@rvsri.com
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|