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Effect of Cell Concentration on the Acylation of Penicillin G Enzymatic Reaction in Immobilized Cells
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نویسنده
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Barin M. ,Otadi M. ,Khorasheh F. ,Kheirolomoom A.
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منبع
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scientia iranica - 2009 - دوره : 16 - شماره : 1 - صفحه:69 -73
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چکیده
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E. coli was immobilized by entrapment within calcium alginate beads using cell suspensionsof different concentrations. the immobilization procedure resulted in beads with a homogeneous cell distribution, referred to as a non-overlapping cell (nc) configuration. if cells within the beads wereallowed to grow, the nc configuration would transform into a clustered cell (cc) configuration as a result of cell growth. enzyme activity and substrate conversion were obtained for nc and cc configuration, ofdifferent cell density, using penicillin g acylation by penicillin g acylase, to produce ti-amino penicillanic acid. enzyme activity and conversion were found to depend on both cell concentration and configuration.beads with nc configuration resulted in higher enzyme activity and conversion compared with cc configurations of similar cell density.
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کلیدواژه
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E-coli; Immobilized cells; Penicillin G acylation; Cell distribution
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آدرس
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islamic azad university, Biotechnology Group, ایران, islamic azad university, Biotechnology Group, ایران, sharif university of technology, Department of Chemical and Petroleum Engineering, ایران, University of California at Davis, Department of Chemical and Petroleum Engineering, USA
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Authors
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