In-Situ Digestion of Serum Samples in Graphite Furnace Prior to Determination by ETAAS

An accurate in-situ digestion method was developed for this approach to separate the traces of pb, mn and zn from their concomitant in serum samples, prior to determination by etaas. pd was used as a modifier to stabilize the analyte from decomposition at 900-1000 (°c). stabilization was much more effective if the mixture modifier and digested sample were first pre-pyrolyzed on the furnace prior to the atomization. determination of pb, mn and zn in serum at a concentration of 0.1 ppb-1.0 ppm was performed by this method. percent relative standard deviations for seven replicates in measurements were found to be 3.4, 3.1 and 4.0, respectively. the accuracy of technique was established by analyzing standard reference materials. the results for determination of the tested elements were within the range of certified values.